DNA sequencing protocols /
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| Pengarang-pengarang Lain: | , |
|---|---|
| Format: | Buku |
| Bahasa: | English |
| Diterbitkan: |
Totowa, N.J. :
Humana Press,
c1993.
|
| Siri: | Methods in molecular biology (Clifton, N.J.) ;
23. |
| Subjek-subjek: | |
| Penanda-penanda: |
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| 008 | 930520s1993 njua b 001 0 eng c | ||
| 010 | |a 93022932 | ||
| 020 | |a 0896032485 | ||
| 035 | 0 | |a ocm28224280 | |
| 040 | |a NLM/DLC |c DLC |d DLC | ||
| 050 | 0 | 0 | |a QP624 |b .D634 1993 |
| 060 | 0 | 0 | |a W1 |b ME9616J v.23 1993 |
| 060 | 1 | 0 | |a QU 58 |b D62965 1993 |
| 082 | 0 | 0 | |a 574.87/3282 |2 20 |
| 245 | 0 | 0 | |a DNA sequencing protocols / |c edited by Hugh G. Griffin and Annette M. Griffin. |
| 260 | |a Totowa, N.J. : |b Humana Press, |c c1993. | ||
| 300 | |a xii, 392 p. : |b ill. ; |c 23 cm. | ||
| 490 | 1 | |a Methods in molecular biology ; |v 23 | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | |a DNA sequencing / Hugh G. Griffin and Annette M. Griffin -- M13 cloning vehicles : their contribution to DNA sequencing / Joachim Messing -- Cloning into M13 / Quinzhong Yu -- Transfection of E. coli with M13 DNA / Fiona M. Tomley -- M13 phage growth and single-strand DNA preparation / Fiona M. Tomley -- M13 phage growth and DNA purification using 96 well microtiter trays / Alan T. Bankier -- Generation of random fragments by sonication / Alan T. Bankier -- Generation of a nested set of deletions using exonuclease III / George Murphy -- Sequential deletions of single-stranded DNA : cyclone sequencing / George Murphy -- Subcloning for DNA sequencing / Gary M. Studnicka [and others] -- Sequencing using custom designed oligonucleotides / Ulrike Gerischer and Peter Durre -- Dideoxy sequencing reactions using Klenow fragment DNA polymerase I / Alan T. Bankier -- Dideoxy sequencing reactions using T7 polymerase / Peter Dürre and Ulrike Gerischer -- | |
| 505 | 0 | |a Dideoxy sequencing reactions using sequenase version 2.0 / Hugh G. Griffin and Annette M. Griffin -- Dideoxy sequencing reactions using Taq polymerase / Fabrizio Arigoni and P. Alexandre Kaminski -- Pouring linear and buffer-gradient sequencing gels / Paul Littlebury -- Electrophoresis of sequence reaction samples / Alan T. Bankier -- Plasmid sequencing / Hugh G. Griffin and Annette M. Griffin -- Plasmid sequencing / George Murphy -- Direct sequencing of inserts cloned into lambda vectors / Afshan N. Malik -- Cosmid sequencing / Molly Craxton -- Genomic sequencing / Gerd P. Pfeifer and Arthur D. Riggs -- Sequencing of double-stranded PCR products / Susannah Gal -- Sequencing double-stranded linear DNA with sequenase and [[alpha]-³⁵S] dATP / Jean-Laurent Casanova -- Solid phase PCR sequencing of biotinylated products / Andrew Green and Mark Vaudin -- Cycle sequencing / Robert W. Blakesley -- Direct blotting electrophoresis / Stephan Beck -- | |
| 505 | 0 | |a Multiplex DNA sequencing / Stephan Beck -- DNA sequencing by chemiluminescent detection / Stephan Beck -- Reverse sequencing of M13 cloned DNA / Alan T. Bankier -- Terminal labeling of DNA for Maxam and Gilbert sequencing / Eran Pichersky -- DNA sequencing by the chemical method / Eran Pichersky -- DNA sequencing by chemical degradation using one, two, and four different fluorophores / André Rosenthal -- Linear amplification sequencing with dye terminators / André Rosenthal and D. Stephen Charnock-Jones -- Sequencing reactions for the Applied Biosystems 373A automated DNA sequencer / Nicolette Halloran, Zijin Du and Richard K. Wilson -- Sequencing reactions for ALF (EMBL) automated DNA sequencer / Wilhelm Ansorge [and others] -- Sequencing using the DuPont Genesis 2000 DNA analysis system / Len Hall -- Use of robotic workstations in DNA sequencing / Alan T. Bankier. | |
| 650 | 0 | |a Nucleotide sequence. | |
| 650 | 2 | |a Sequence Analysis, DNA. | |
| 700 | 1 | |a Griffin, Hugh G. | |
| 700 | 1 | |a Griffin, Annette M. | |
| 830 | 0 | |a Methods in molecular biology (Clifton, N.J.) ; |v 23. | |
| 988 | |a 20020608 | ||
| 906 | |0 OCLC | ||