Experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis

A lipid acyl hydrolase (LAH) enzyme has been purified from skim latex serum, a waste product of Hevea brasiliensis, using DEAE Sepharose™ Fast Flow ion exchange chromatography. The ~45kDa active LAH was expressed by spectrophotometric study and SDS-PAGE. One-Factor-at-A-Time (OFAT) method and Centra...

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Main Authors: Mohamed, Nazhirah, Yusof, Faridah
格式: Article
语言:English
English
出版: AENSI Publications 2014
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在线阅读:http://irep.iium.edu.my/39221/
http://irep.iium.edu.my/39221/
http://irep.iium.edu.my/39221/1/P_46.pdf
http://irep.iium.edu.my/39221/4/39221_Experimental%20design%20and%20statistical_scopus.pdf
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