Technical improvement in DNA preparation in pulsed-field gel electrophoresis
We report a rapid pulsed-field gel electrophoresis (PFGE) protocol for subtyping Salmonella isolates. Modifications were made to reduce the number of steps and duration of proteolysis in our original protocol for the preparation of intact, genomic DNA for PFGE analysis. Comparison of PFGE patterns b...
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| Main Authors: | , , |
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| Format: | Article |
| Published: |
2003
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/9949/ http://eprints.um.edu.my/9949/1/Thong%2C_Lai%2C_Goh%2C_2003.pdf |
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| Summary: | We report a rapid pulsed-field gel electrophoresis (PFGE) protocol for subtyping Salmonella isolates. Modifications were made to reduce the number of steps and duration of proteolysis in our original protocol for the preparation of intact, genomic DNA for PFGE analysis. Comparison of PFGE patterns by independent researchers showed that subtyping results using this modified, rapid protocol were highly reproducible. |
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