Discrimination of probiotic Lactobacillus strains for poultry by repetitive sequenced‐based PCR fingerprinting
BACKGROUND: Four repetitive element sequence‐based polymerase chain reaction (rep‐PCR) methods, namely repetitive extragenic palindromic PCR (REP‐PCR), enterobacterial repetitive intergenic consensus PCR (ERIC‐PCR), polytrinucleotide (GTG)5‐PCR and BOX‐PCR, were evaluated for the molecular different...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Published: |
Society of Chemical Industry
2012
|
| Subjects: | |
| Online Access: | https://doi.org/10.1002/jsfa.4627 https://doi.org/10.1002/jsfa.4627 http://umpir.ump.edu.my/20012/1/Discrimination%20of%20probiotic%20Lactobacillus%20strains%20for%20poultry-fist-2011-1.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | BACKGROUND: Four repetitive element sequence‐based polymerase chain reaction (rep‐PCR) methods, namely repetitive extragenic palindromic PCR (REP‐PCR), enterobacterial repetitive intergenic consensus PCR (ERIC‐PCR), polytrinucleotide (GTG)5‐PCR and BOX‐PCR, were evaluated for the molecular differentiation of 12 probiotic Lactobacillus strains previously isolated from the gastrointestinal tract of chickens and used as a multistrain probiotic. This study represents the first analysis of the comparative efficacy of these four rep‐PCR methods and their combination (composite rep‐PCR) in the molecular typing of Lactobacillus strains based on a discriminatory index (D). |
|---|