Polymorphisms of x-ray repair cross-complementing group 1 (xrcc1) in gastrointestinal cancer
This research is done to identify the polymorphisms of x-ray repair crosscomplementing group I (XRCC 1) gene in gastrointestinal (GI) cancer. DNA was isolated from the blood samples, which were taken from Hospital Queen Elizabeth. The quality of DNA isolated was checked by spectrophotometer and we...
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| Main Author: | |
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| Format: | Academic Exercise |
| Language: | English |
| Published: |
2008
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| Subjects: | |
| Online Access: | http://eprints.ums.edu.my/5909/ http://eprints.ums.edu.my/5909/1/ae0000000921.pdf |
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| Summary: | This research is done to identify the polymorphisms of x-ray repair crosscomplementing
group I (XRCC 1) gene in gastrointestinal (GI) cancer. DNA was isolated from the blood samples, which were taken from Hospital Queen Elizabeth.
The quality of DNA isolated was checked by spectrophotometer and were used for
polymerase chain reaction. Polymerase chain reaction was carried out using a pair
of primer specifically to amplify 517bp of the XRCC 1 gene that contained the
Arg399G1n site. After the PCR, the amplified DNA was cut by using specific
enzyme which will only cut at specific sequence of the DNA (Bcnl). After the
restriction enzyme digestion, the result of restriction enzyme digestion was
analyzed by using agarose gel electrophoresis in order to determine whether there is
any polymorphism in the region of interest. From the result of the agarose gel
electrophoresis, out of 10 samples, four samples with genotype frequency of 0.3481
were homozygote wild-type (Arg/Arg), three samples with genotype frequency of
0.17 were homozygote variant (Gln/Gln), and three samples with genotype
frequency of 0.48 were heterozygote (Arg/Gln). However, this is just a preliminary
result because we are not able to get large number of samples. |
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