HEXAVALENT CHROMIUM REDUCTION BY Acinetobacter haemolyticus USING AGRICULTURAL WASTE
The high cost of culture growth medium is one of the problems faced in the scaling-up of biological processes involved in wastewater treatment. This makes it imperative to find a useful, cheap and easily available alternative source for culture growth medium. The possibility of using sugarcane ba...
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| தலைமை எழà¯à®¤à¯à®¤à®¾à®³à®°à¯: | |
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| வடிவமà¯: | Thesis |
| வெளியீடபà¯à®ªà®Ÿà¯à®Ÿà®¤à¯: |
2013
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| பகà¯à®¤à®¿à®•à®³à¯: | |
| நிகழà¯à®¨à®¿à®²à¯ˆ அணà¯à®•à®²à¯: | http://pustaka2.upsi.edu.my/eprints/649/ http://pustaka2.upsi.edu.my/eprints/649/1/Hexavalent%20Chromium%20Reduction%20By%20Actnetobacter%20Haemolyticus%20Using%20Agricultural%20Waste.pdf |
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| தொகà¯à®ªà¯à®ªà¯: | The high cost of culture growth medium is one of the problems faced in the
scaling-up of biological processes involved in wastewater treatment. This makes it
imperative to find a useful, cheap and easily available alternative source for culture
growth medium. The possibility of using sugarcane bagasse (SCB), solid pineapple
waste (SPW) and soybean meal (SBM) as alternative sources for culture medium is
preferable as these agricultural wastes are easily available, cheap and abundantly
grown. The present work highlights the use of SCB, SPW and SBM to sustain the
bacterial population for the Cr(VI) reduction process. Growth of A. haemolyticus in
agricultural wastes was measured by optical density (00600) followed by viable cell
counts. Reduction of Cr(VI) was determined using diphenylcarbazide method. For
all Cr(VI) concentrations tested (10-100 mg/L), SCB-adapted A. haemolyticus
showed the highest reduction ranging from 92-99% followed by SPW and SBM
with 40-94% and 21-85% reduction respectively. From the FESEM-EOX analysis,
toxicity effect can be seen clearly from the shape of bacteria in the presence of 100
mg/L Cr(VI). The FT-IR analysis showed shifting of the C-O band absorption peak
from 1252-1261 ern" and 1048-1037 cm-I after Cr-Ioaded which was due to the
binding of Cr(VI) to this functional group. In this study, down-ward biofilm
packed-bed reactor was used. A minimum of 4 h was required for complete
reduction of Cr(VI) to Cr(III) at the flow rate of 3.0 mUmin using 25 mg/L initial
Cr(VI) concentration. Cr(Vl) reduction mechanism study using XPS and ESR
implies that the Cr bound to the SCB and SCB-adapted A. haemolyticus were mostly
in trivalent form. SCB can serve as an alternative and cost-effective growth medium
for cultivation of A. haemolyticus with high percent reduction of Cr(VI).
Phylogenetic analysis revealed that the microbial community was dominated by
Chitinophaga terrae, Laribacter ho ngkongensis, Ottowia thiooxydans, Rhizobium
cellulosilyticum, Candidate division OPIO, Pedobacter sp. and uncultured bacterium. |
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