Isolation and optimization of napthalene degradative bacteria
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous in the environment and cause great environmental concern because of their persistent, toxicity, mutagenicity and carcinogenicity. Therefore they are listed by the United States Environmental Protection Agency (USEPA) as priority pollutants. The...
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| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Published: |
2009
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| Subjects: | |
| Online Access: | http://eprints.uthm.edu.my/2019/ http://eprints.uthm.edu.my/2019/1/ISOLATION_AND_OPTIMIZATION_OF_NAPTHALENE_NORZILA_OTHMAN_2009.pdf |
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| Summary: | Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous in the environment and cause great
environmental concern because of their persistent, toxicity, mutagenicity and carcinogenicity.
Therefore they are listed by the United States Environmental Protection Agency (USEPA) as
priority pollutants. The principle process for the successful removal and elimination of PAHs
from contaminated environment is microbial degradation. Many studies from temperate
countries had reported on biodegradation of PAHs but limited information could be found on
tropical region. This study is carried out to isolate PAH degradative bacteria from municipal
sludge and to optimize their degradation condition including pH, temperature and napthalene
concentration required for bioremediation purposes. Several bacterial strains were isolated
through enrichment and one strain, Micrococcus diversus that was tentatively identified by
the Biolog system, demonstrated a high removal rate of naphthalene over other strains.
Following one day lag phase more than 85% of naphthalene degraded after six days
incubation. Maximum rate of naphthalene removal occurred in the culture containing 100
mgU' of naphthalene. Media at a pH 7.0 was more favourable for the degradation of
napthalene by Micrococcus diversus. Optimal temperature was determined as 30°C. The
isolated Micrococcus diversus demonstrated to be a feasible strain for degradation of
napthalene at a neutral pH, 30°C even up to a napthalene concentration of 100 mgL'1. |
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