Widefield surface plasmon resonance microscope: a novel biosensor study of cell attachment to micropatterned substrates
In this study, Human adult, low Ca2+, high Temperature (HaCaTs) Keratinocytes were cultured on microcontact printed fibronectin repeat gratings of 1.8, 3.8, 5, 12.5 and 25μm for 24 hours. The cells were then fixed with 0.1% Glutaraldehyde and dehydrated in serial alcohol [1]. The alignment of the ce...
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| Main Authors: | , , , , , , |
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| Format: | Conference or Workshop Item |
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| Online Access: | http://eprints.uthm.edu.my/2617/ http://eprints.uthm.edu.my/2617/1/9.pdf |
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| Summary: | In this study, Human adult, low Ca2+, high
Temperature (HaCaTs) Keratinocytes were cultured on microcontact
printed fibronectin repeat gratings of 1.8, 3.8, 5, 12.5
and 25μm for 24 hours. The cells were then fixed with 0.1%
Glutaraldehyde and dehydrated in serial alcohol [1]. The
alignment of the cells were then measured, where 0º represents
100% alignment to the pattern, in order to identify those features
that promoted the highest degree of cell alignment. From
the quantitative analysis it became clear that HaCaTs cells
align most readily to the 12.5μm pattern. A 12.5μm stamp was
therefore used to stamp pattern fibronectin on to prefabricated
Au/Cr/glass surface plasmon substrates. HaCaTs cells
were cultured on the substrates for 24 hours and imaged with
the “Widefield Surface Plasmon Resonance” (WSPR) microscope
[1]. The WSPR system enables the examination of
nanometric interfacial interactions of HaCaTs on patterned
and un-patterned surface at lateral resolution down to 500nm.
Our preliminary results demonstrate that the WSPR microscope
is capable of imaging the cell surface interface and as
such will prove to be a very useful tool in understanding the
processes involved in cell guidance and wound repair. |
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