High resolution imaging of TGFΒ3 treated human keratinocyte via a newly developed widefield surface plasmon resonance microscope
In this study high resolution imaging of human adult (HaCaTs) Keratinocytes cells using the newly developed Widefield Surface Plasmon Resonance (WSPR) system will be discussed. Surface Plasmon Resonance (SPR) occurs at the interface between a dielectric and a thin conducting layer when p-polarized l...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Conference or Workshop Item |
| Subjects: | |
| Online Access: | http://eprints.uthm.edu.my/2618/ http://eprints.uthm.edu.my/2618/1/26.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | In this study high resolution imaging of human
adult (HaCaTs) Keratinocytes cells using the newly developed
Widefield Surface Plasmon Resonance (WSPR) system will be
discussed. Surface Plasmon Resonance (SPR) occurs at the
interface between a dielectric and a thin conducting layer
when p-polarized light strikes at a specific angle thus excites
free electrons and generates surface plasmon electromagnetic
wave. The SPR excitation angle can be changed by the binding
of bio-molecular species to the metallised layer, and is directly
proportional to the refractive index and thickness of that molecular
species. Our WSPR system provide high lateral resolution
imaging close to 500 nanometers [1] and was used to investigate
cell surface interactions under two different culture
conditions: HaCaTs cultured on SPR substrate with Transforming
Growth Factor β3 (TGFβ3) (50ng/ml) [2] and without
TGFβ3. In less than 24 hours, HaCaTs cultured in the presence
of TGFβ3 showed enhanced division and motility along
with decreased cell attachment as compared with cells maintained
in TGFβ3 free media. It is to be noted that cellular
signaling by TGFβ3 is very important for enhancing tissue
development in wound repair and that this study for the first
time enabled optical interrogation of cell surface interface
without the need for Immunostaining. |
|---|