Enumeration of salmonella in compost material by a nonculture based method
Accurate enumeration of Salmonella spp. is important for assessing whether this pathogen has survived composting. Recent literature has reported that enumeration of Salmonella spp. using standard microbiological methods has a numbers of disadvantages, particularly the time taken to obtain a result....
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| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Published: |
2009
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| Subjects: | |
| Online Access: | http://eprints.uthm.edu.my/5335/ http://eprints.uthm.edu.my/5335/1/151_p_Sunar.pdf |
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| Summary: | Accurate enumeration of Salmonella spp. is important for assessing whether this
pathogen has survived composting. Recent literature has reported that enumeration of Salmonella
spp. using standard microbiological methods has a numbers of disadvantages, particularly the
time taken to obtain a result. This research is an attempt to develop a rapid, low-cost detection
method that is quantitative, highly sensitive and target specific. This paper reports the
development of a DNA fragment that can be used to quantify Salmonella spp. by competitive
polymerase chain reaction (cPCR) targeted at the invA gene of Salmonella (PCR primers that
target the invA gene are reported to have very high specificity for Salmonella strains). It is shown
that cPCR, which could be completed in 5 hours, could quantify the number of copies of the
Salmonella invA gene in a sample solution. |
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