Effects of the immobilization of recombinant Escherichia coli on cyclodextrin glucanotransferase (CGTase) excretion and cell viability
The excretion of recombinant enzymes is a preferred approach for protein expression because of the associated high level of expression, low level of proteolysis, ease of purification andmorefavorable folding environment. However, cell lysis is one of the major drawbacks in the excretion of enzymes w...
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| Main Authors: | , , , , |
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| Format: | Article |
| Published: |
Elsevier
2015
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1016/j.bej.2015.02.013 http://dx.doi.org/10.1016/j.bej.2015.02.013 http://eprints.uthm.edu.my/8093/1/siti_fatimah_zaharah.pdf |
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| Summary: | The excretion of recombinant enzymes is a preferred approach for protein expression because of the
associated high level of expression, low level of proteolysis, ease of purification andmorefavorable folding
environment. However, cell lysis is one of the major drawbacks in the excretion of enzymes when using
Escherichia coli as a host. In this study, the effects of different polymer of hollow fiber membrane and
culture conditions on the enzyme excretion, cell lysis and plasmid stability of immobilized E. coli were
investigated. The cells immobilized on a hollow fiber membrane composed of a polyvinylidene fluoride
(PVDF) polymer exhibited a 2–4-fold increase in CGTase excretion, over a 100% increase in plasmid
stability and 28-60% reduction in cell lysis compared with free cells. Hence, the immobilization of E. coli
using a hollow fiber membrane was demonstrated to increase enzyme excretion and cell stability. |
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