Immobilization of horseradish peroxidase onto polyaniline nanoparticle monolayer
Enzyme have been covalently bonded to a wide variety of water- imoluble polymeric supports. Recently, the class of organic polymers called ' conductive polymers' has attracted considerable attention and has been used as supports for enzyme immobilization. Among these polyaniline emeraldine...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Published: |
IDOSI Publications
2010
|
| Subjects: | |
| Online Access: | http://eprints.uthm.edu.my/8310/ http://eprints.uthm.edu.my/8310/1/4.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Enzyme have been covalently bonded to a wide variety of water- imoluble polymeric supports.
Recently, the class of organic polymers called ' conductive polymers' has attracted considerable attention and
has been used as supports for enzyme immobilization. Among these polyaniline emeraldine base (PANI-EB)
occupies an important place because of its unique propehes, including good environmental stability, the
simplicity of doping process and its excellent redox recyclability enabling polymers with significant differences
to be constructed by means of simple acidic or basic treatments. This study intends to see the effect and
properties Horseradsh Peroxidase (HRP) when it is deposited onto PANI-EB nanoparticle monolayer by using
electrodeposition techmque. During electrodeposition, HRF will be deposited on PAM-EB monolayer that act
as a cathode. For UV- visible spectnun the PAN-EB with and without HRF shows two sharp absorption peaks
at -3201x11 and -800nm. HRF helps in increasing the conductivity of PANI-EB. Thls support by a resistivity
value obtained from four point probe measurement. Uniformity during electrodeposition can be seen from
VPSEM result. As a conclusion, immobilization of enzyme onto PANI-EB can helps in increasing the
conductivity properties of PANI-EB. |
|---|