Identification of bacteria and the effect on compressive strength of concrete
This paper presents the species of bacteria used in this study as well as the effect of the bacteria on compressive strength of bioconcrete. Bioconcrete is not only more environmentally friendly but it is easy to procure. The objective of this research is to identify the ureolytic bacteria and sulph...
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| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Published: |
2015
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| Subjects: | |
| Online Access: | http://eprints.uthm.edu.my/8456/ |
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| Summary: | This paper presents the species of bacteria used in this study as well as the effect of
the bacteria on compressive strength of bioconcrete. Bioconcrete is not only more
environmentally friendly but it is easy to procure. The objective of this research is to identify
the ureolytic bacteria and sulphate reduction bacteria that have been isolated and further use the
bacteria in concrete to determine the effect of bacteria on compressive strength. Identification
of bacteria is conducted through Polymerase chain reaction (PCR) method and DNA
sequencing. The DNA of the bacteria was run through BLAST algorithm to determine the
bacterial species.The bacteria were added into the concrete mix as a partial replacement of
water. 3% of water is replaced by ureolytic bacteria and 5% of water is replaced by sulphate
reduction bacteria. After running BLAST algorithm the bacteria were identified as
Enterococcus faecalis (ureolytic bacteria) and Bacillus sp (sulphate reduction bacteria). The
result of the compressive strength for control is 36.0 Mpa. Partial replacement of 3% water by
ureolytic bacteria has strength of 38.2Mpa while partial replacement of 5% of water by
sulphate reduction bacteria has strength of 42.5Mpa. The significant increase of compressive
strength with the addition of bacteria shows that bacteria play a significant role in the
improvement of compressive strength. |
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