Isolation of sulphate reduction bacteria (SRB) to improve compress strength and water penetration of bio-concrete
The objective of this study is to isolate sulphate reduction bacteria (SRB) from acid mire water collected at Sg Pelepah Kota Tinggi, Johor Malaysia. The isolation process was conducted in high alkaline and anaerobic conditions to sustain the bacteria in concrete environment. Properties tests such a...
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| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Published: |
2015
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| Subjects: | |
| Online Access: | http://eprints.uthm.edu.my/8458/ |
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| Summary: | The objective of this study is to isolate sulphate reduction bacteria (SRB) from acid
mire water collected at Sg Pelepah Kota Tinggi, Johor Malaysia. The isolation process was
conducted in high alkaline and anaerobic conditions to sustain the bacteria in concrete
environment. Properties tests such as compressive strength and water penetration were
conducted. The result showed that optimal growth condition of sulphate reduction bacteria is
pH 9-10. It was also observed that the bacteria is a coccus shape after gram staining process.
The bacteria was used after 10 days of culturing prior to growth curve measurement. The liquid
culture containing sulphate reduction bacteria were used at 1%, 3% and 5% as replacement
ratio of water content. Concrete specimens were cured in the air conditions for 7, 14 and 28
days. Maximum increment on compressive strength was 13.0% and decrement in water
penetration was 8.5% occurred with 5% of SRB. The enhancement in compressive strength and
water penetration performance was due to calcium precipitation within concrete pores. Image
of scanning electronic microscopy (SEM) showed bacteria sustained and survived in concrete
environment by reducing diameter of pores in concrete specimens. |
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