Transforming growth factor- β1 and β3 manipulation of HaCaT keratinocyte attachment to a 12.5μm fibronectin patterned surface illustrated by widefield surface plasmon resonance microscopy
The influence of Transforming Growth Factor- β1 (TGF) and β3 on keratinocyte surface attachment has yet to be fully elucidated. Widefield Surface Plasmon Resonance (WSPR) microscopy allows changes in the arrangement of focal contacts to be visualised. HaCaT keratinocytes were plated on a 12.5μm fibr...
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| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Published: |
2008
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| Subjects: | |
| Online Access: | https://link.springer.com/chapter/10.1007/978-3-540-69139-6_27 https://link.springer.com/chapter/10.1007/978-3-540-69139-6_27 |
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| Summary: | The influence of Transforming Growth Factor-
β1 (TGF) and β3 on keratinocyte surface attachment has yet to
be fully elucidated. Widefield Surface Plasmon Resonance
(WSPR) microscopy allows changes in the arrangement of
focal contacts to be visualised. HaCaT keratinocytes were
plated on a 12.5μm fibronectin micropattern with TGF-β1 or
β3 and WSPR photomicrographs were taken. The effects of
TGF-β3 inhibition was attenuated by the 12.5μm fibronectin
micropattern. TGF-β1 promoted an arrangement of focal
contacts consistent with both stationary and migratory HaCaT
keratinocyte phenotypes. The arrangement and location of the
focal contacts supports literature suggesting TGF-β3 promotes
a migratory phenotype whilst TGF-β1 in excess impairs reepithelialisation. |
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