Cloning of influenza B NS1 gene in Escherichia Coli
The non-structural NS1 protein of influenza B virus is a multifunctional virulence protein which is involved in the transport of viral RNA. Inside the host cell, NS1B antagonizes and inhibits the a/ß interferon system which is induced as host antiviral response. Moreover, it prevents the activation...
Saved in:
| 主要作者: | |
|---|---|
| 格式: | Thesis |
| 语言: | English |
| 出版: |
2010
|
| 主题: | |
| 在线阅读: | http://eprints.utm.my/12283/ http://eprints.utm.my/12283/4/AliAdelDawoodMFBB2010.pdf |
| 标签: |
添加标签
没有标签, 成为第一个标记此记录!
|
| 总结: | The non-structural NS1 protein of influenza B virus is a multifunctional virulence protein which is involved in the transport of viral RNA. Inside the host cell, NS1B antagonizes and inhibits the a/ß interferon system which is induced as host antiviral response. Moreover, it prevents the activation of double-stranded-RNA activated protein kinase (PKR) by binding to dsRNA and inhibits the maturation of GAS8 (gene of tumor suppressor). NS1 protein is utilized as a target for diagnostic of influenza viruses in infected animals. pUC57 carrying NS1 synthetic gene of influenza B was attempted to be transformed into Escherichia coli strain BL21(DE3). NS1B was extracted and attempted to be cloned into prokaryotic expression vectors pET-32b, pET-32a, pQE-81L and pQE-80L, respectively using restriction digestion enzymes (SacI, PstI and HindIII). Then, recombinant DNA was attempted to be transformed into Escherichia coli strains BL21(DE3) and DH5a. |
|---|