Determination of effective diffusion coeficient of immobilized baker's yeast invertase in various concentration of PVA-alginate matrix
Baker's yeast invertase is immobilizzed in PVA-alginate matrix using an improved method PVA beads were prepared by adding calcium alginate to improve its stability, mechanical and chemical properties. Boric acid was used as the cross-linking agent and additional chemicals consisteing of 10% bor...
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| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Published: |
2007
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| Subjects: | |
| Online Access: | http://eprints.utm.my/24444/ |
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| Summary: | Baker's yeast invertase is immobilizzed in PVA-alginate matrix using an improved method PVA beads were prepared by adding calcium alginate to improve its stability, mechanical and chemical properties. Boric acid was used as the cross-linking agent and additional chemicals consisteing of 10% boric acid and sodium sulphate solution was used as a treatment solution to harden the PVA-alginate beads. The determination of the effective diffusion of PVA-alginate matrix is the vital step in optimizing the preparation of immobilized and water-soluble biocatalyst. In the study the two-level full factorial design was used to investigate the effect of PVA and boric acid concentrations on diffusion coefficient. |
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