High temperature high performance liquid chromatography of triazole fungicides on carbon-clad zirconia stationary phase
Temperature is an important separation parameter in reversed-phase high performance liquid chromatography. In this work, high-temperature high performance liquid chromatography on a carbon-clad zirconia column using water-rich eluent is evaluated as a new approach for the separation of selected tria...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Faculty of Science, Universiti Teknologi Malaysia
2004
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| Subjects: | |
| Online Access: | http://eprints.utm.my/28191/ http://eprints.utm.my/28191/1/SeeHongHeng2004_HighTemperatureHighPerformanceLiquid.pdf |
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| Summary: | Temperature is an important separation parameter in reversed-phase high performance liquid chromatography. In this work, high-temperature high performance liquid chromatography on a carbon-clad zirconia column using water-rich eluent is evaluated as a new approach for the separation of selected triazole fungicides. The selectivity and retention patterns of triazole fungicides, hexaconazole, tebuconazole, propiconazole, and difenoconazole were investigated at temperatures of 100 °C to 150 °C. Excellent separations were achieved for propiconazole, tebuconazole and difenoconazole under all conditions studied. Despite insufficient separations between hexaconazole and propiconazole, slight separations were observed for the cis and trans propiconazole isomers. Van’t Hoff plots for the separations were linear suggesting that no changes occurred in the retention mechanism for the selected fungicides over the temperature range studied.
Suhu merupakan parameter penting dalam kromatografi cecair prestasi tinggi fasa terbalik. Dalam kajian ini, kromatografi cecair prestasi tinggi bersuhu tinggi dengan turus zirkonia tersalut karbon menggunakan pengelusi kaya-air telah dikaji sebagai suatu pendekatan baru bagi pemisahan racun rumpai triazola. Kepilihan dan pola penahanan rumpai triazola heksakonazola, tebukonazola, propikonazola, and difenokonazola telah dikaji pada suhu 100 °C hingga 150 °C. Pemisahan yang sangat baik diperoleh bagi propikonazola, tebukonazola, dan difenokonazola pada semua keadaan kajian. Sementara tiada pemisahan yang lengkap diperoleh bagi heksakonazola dan propikonazola, sedikit pemisahan diperoleh bagi pasangan cis dan trans bagi propikonazola. Lakaran Van’t Hoff bagi pemisahan tersebut adalah linear yang menandakan tiada perubahan berlaku pada mekanisme penahanan bagi rancun rumpai terpilih berkenaan pada julat suhu kajian. |
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