Facile modulation of enantioselectivity of thermophilic Geobacillus zalihae lipase by regulating hydrophobicity of its Q114 oxyanion
Site-directed mutagenesis of the oxyanion-containing amino acid Q114 in the recombinant thermophilic T1 lipase previously isolated from Geobacillus zalihae was performed to elucidate its role in the enzyme's enantioselectivity and reactivity. Substitution of Q114 with a hydrophobic methionine t...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Published: |
Elsevier Inc.
2016
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| Subjects: | |
| Online Access: | http://eprints.utm.my/71955/ http://eprints.utm.my/71955/ |
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