Reductive alkylation of lipase: experimental and molecular modeling approaches
Candida rugosa lipase was modified via reductive alkylation to increase its hydrophobicity to work better in organic solvents. The free amino group of lysines was alkylated using propionaldehyde with different degrees of modification obtained (49 and 86%). Far-ultraviolet circular dichroism (CD) spe...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Published: |
2004
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| Subjects: | |
| Online Access: | http://www.scopus.com/inward/record.url?eid=2-s2.0-4344684591&partnerID=40&md5=eaa7316f4dcf1404d26a1ee93a5b2340 http://www.scopus.com/inward/record.url?eid=2-s2.0-4344684591&partnerID=40&md5=eaa7316f4dcf1404d26a1ee93a5b2340 |
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