Physical and stability characteristics of Burkholderia cepacia lipase encapsulated in κ-carrageenan
In this work, a novel approach for lipase immobilization was exploited. Lipase from Burkholderia cepacia was encapsulated into κ-carrageenan by co-extrusion method to form a liquid core capsule. The diameter of the encapsulated lipase was found to be in the range of 1.3-1.8 mm with an average membr...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2009
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| Subjects: | |
| Online Access: | http://eprints.ums.edu.my/1576/ http://eprints.ums.edu.my/1576/ http://eprints.ums.edu.my/1576/ http://eprints.ums.edu.my/1576/1/Physical_and_stability_characteristics_of_Burkholderia_cepacia_lipase_encapsulated_in_%C3%8E%C2%BA-carrageenan.pdf |
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| Summary: | In this work, a novel approach for lipase immobilization was exploited. Lipase from Burkholderia cepacia was encapsulated into κ-carrageenan by co-extrusion method to form a liquid core capsule. The diameter of the encapsulated lipase was found to be in the range of 1.3-1.8 mm with an average membrane thickness of 200 μm and 5% coefficient of variance. The encapsulation efficiency was 42.6% and 97% moisture content respectively. The encapsulated lipase was stable between pH 6 and 9 and temperature until 50 °C. The encapsulated lipase was stable until disintegration of the carrier when stored at 27 °C and retained 72.3% of its original activity after 6 cycles of hydrolysis of p-NPP. The encapsulated lipase was stable in various organic solvents including methanol, ethanol, iso-propanol, n-hexane and n-heptane. Kinetic parameters Km and Vmax were found to be 0.22 mM and 0.06 μmol/min for free lipase and 0.25 mM and 0.05 μmol/min for encapsulated lipase respectively. © 2008 Elsevier B.V. All rights reserved. |
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