Characterizing the interfacial topology of cells attached to liquid crystals
Widefield surface plasmon resonance (WSPR) microscope provides high resolution imaging of the cell-substrate interface. We report the application of our WSPR imaging system to study the interaction of keratinocytes with the liquid crystals (LC). Imaging of the fixed cell-LC-gold and fixed cellgold i...
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| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Published: |
2012
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| Subjects: | |
| Online Access: | http://eprints.uthm.edu.my/6121/ http://eprints.uthm.edu.my/6121/1/Characterizing_the_Interfacial_Topology.pdf |
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| Summary: | Widefield surface plasmon resonance (WSPR)
microscope provides high resolution imaging of the cell-substrate
interface. We report the application of our WSPR imaging
system to study the interaction of keratinocytes with the liquid
crystals (LC). Imaging of the fixed cell-LC-gold and fixed cellgold
interfaces were performed in air using a 1.45 NA objective
based system. In the WSPR microscopy technique, keratinocytes
were found forming multiple narrow concentric rings with the
presence of liquid crystals but these cells expressed wide band of
contact area when they are directly cultured on the glass surface.
Our work showed the feasibility of using WSPR microscopy
system in imaging cells interaction with liquid crystals. |
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