Saccharification of polysaccharide content of palm kernel cake using enzymatic catalysis for production of biobutanol in acetone-butanol-ethanol fermentation
In this work, hydrolysis of cellulose and hemicellulose content of palm kernel cake (PKC) by different types of hydrolytic enzymes was studied to evaluate monomeric sugars released for production of biobutanol by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) in acetone-butanol-ethanol (AB...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Published: |
Elsevier Ltd
2016
|
| Subjects: | |
| Online Access: | http://eprints.utm.my/73931/ http://eprints.utm.my/73931/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | In this work, hydrolysis of cellulose and hemicellulose content of palm kernel cake (PKC) by different types of hydrolytic enzymes was studied to evaluate monomeric sugars released for production of biobutanol by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) in acetone-butanol-ethanol (ABE) fermentation. Experimental results revealed that when PKC was hydrolyzed by mixed β-glucosidase, cellulase and mannanase, a total simple sugars of 87.81 ± 4.78 g/L were produced, which resulted in 3.75 ± 0.18 g/L butanol and 6.44 ± 0.43 g/L ABE at 168 h fermentation. In order to increase saccharolytic efficiency of enzymatic treatment, PKC was pretreated by liquid hot water before performing enzymatic hydrolysis. Test results showed that total reducing sugars were enhanced to 97.81 ± 1.29 g/L with elevated production of butanol and ABE up to 4.15 ± 1.18 and 7.12 ± 2.06 g/L, respectively which represented an A:B:E ratio of 7:11:1. |
|---|